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Endogenous protein purification

Started by Nefthys, May 27 2009 08:16 AM

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#1 Nefthys

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Posted 27 May 2009 - 08:16 AM

Hi!

I want to perform a kinase assay for an endogenous protein. For tagged proteins I always used immunoprecipitation with beads coupled to the tag but, how can I purify untagged protein from whole cell lysates? Has anyone experience with this?
Should I used affinity chromatography, HLPC...?
Thanks!

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#2 madrius1

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Posted 27 May 2009 - 08:45 AM

How about an IP with specific antibody?

Works pretty well!

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#3 Nefthys

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Posted 28 May 2009 - 12:52 AM

madrius1, on May 27 2009, 08:45 AM, said:

How about an IP with specific antibody?

Works pretty well!

That's the problem, there are no very good antibodies for this protein, so I will try that, but I would need to have in mind some other alternatives.

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