Hi!
I want to perform a kinase assay for an endogenous protein. For tagged proteins I always used immunoprecipitation with beads coupled to the tag but, how can I purify untagged protein from whole cell lysates? Has anyone experience with this?
Should I used affinity chromatography, HLPC...?
Thanks!
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2 replies to this topic
#2
madrius1
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Posted 27 May 2009 - 08:45 AM
How about an IP with specific antibody?
Works pretty well!
Works pretty well!
#3
Nefthys
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Posted 28 May 2009 - 12:52 AM
How about an IP with specific antibody?
Works pretty well!
That's the problem, there are no very good antibodies for this protein, so I will try that, but I would need to have in mind some other alternatives.
