Hi!
I want to perform a kinase assay for an endogenous protein. For tagged proteins I always used immunoprecipitation with beads coupled to the tag but, how can I purify untagged protein from whole cell lysates? Has anyone experience with this?
Should I used affinity chromatography, HLPC...?
Thanks!
0
2 replies to this topic
#2
-
- Active Members
-
- 124 posts
Veteran
Posted 27 May 2009 - 08:45 AM
How about an IP with specific antibody?
Works pretty well!
Works pretty well!
#3
-
- Members
-
- 4 posts
member
Posted 28 May 2009 - 12:52 AM
madrius1, on May 27 2009, 08:45 AM, said:
How about an IP with specific antibody?
Works pretty well!
Works pretty well!
That's the problem, there are no very good antibodies for this protein, so I will try that, but I would need to have in mind some other alternatives.
