1 reply to this topic

[nemo]

Hi there,
Just wondering what kind of bond is set between the primary and the secondary antibody (in western blot or immunocitochemistry). And why that bond is not destroyed when washed with TBS plus tween.
Thank you!!

[Amy831]

Non-covalent interactions occur between antibody and antigen, thus, similarly between primary and secondary antibody, it would seem.

Edited by Amy831, 05 June 2009 - 07:35 PM.