2 replies to this topic

[blake.r]

I was using a 96 well flexible roundbottom plate and getting very high positive readings of around 3 (abs).  I switched to flat bottom, high-binding strip plates and now my absorbance for my positives has dropped to around 2.  

Any help on the loss of OD?

[tinaking]

It is very  normal,  different materials plates have different bingding capacity, so have different OD. If you want have stabilized results,maybe you should use the same material's plate in your experience always.

[sgt4boston]

Elisa flat bottom nunc or corning