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ELISA Optimization


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#1 blake.r

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Posted 27 May 2009 - 05:16 AM

I was using a 96 well flexible roundbottom plate and getting very high positive readings of around 3 (abs). I switched to flat bottom, high-binding strip plates and now my absorbance for my positives has dropped to around 2.

Any help on the loss of OD?

#2 tinaking

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Posted 31 July 2009 - 08:41 PM

It is very normal, different materials plates have different bingding capacity, so have different OD. If you want have stabilized results,maybe you should use the same material's plate in your experience always.

#3 sgt4boston

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Posted 03 August 2009 - 06:41 AM

Elisa flat bottom nunc or corning




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