cloning miRNA library
Posted 27 May 2009 - 03:01 AM
However, I have no prior experience with miRNA methods.
After doing some readings it is my understanding that there are two main strategies for cloning miRNA: 1. the adaptor ligation
2. using polyadenilation and polyT-adaptor primer for RT reaction.
Which one is better in regards to efficiency?
Which one is easier?
I would greatly appreciate if someone could give me a protocol that works.
Thanks to all
Posted 27 May 2009 - 06:16 PM
Posted 28 May 2009 - 06:43 AM
Posted 01 June 2009 - 12:40 AM
Thank you for your former answers- so I started looking into this and I'm currently checking costs. So my next question is- for high throughput sequncing- how many biological repeats do I need? and for the microarray?
Posted 01 June 2009 - 12:47 PM
For array, it's up to you to do duplicate, triplicate, or more........