17 replies to this topic

[mdfenko]

i made an error in my description of the gel we used to separate the myosin isoforms (forgive me, we published in 1983 and i haven't used it since).

the gel was 3.2-5% acrylamide with a 0-8M urea gradient. the stack was 2.8%.

i will pm you with the paper and two supporting papers.

[distazio]

thanks you are really nice! i'll ready the papers. thank u.

i have another question: is it possible to obtain a specific protein cleavage?? and how??

mt

[mdfenko]

you can get a sort of specific cleavage. proteases will cleave at specific residues. but, you usually find several cleavage sites on your proteins.

in some cases you can introduce a specific site that is unique to a specific protease (eg thrombin) and is not otherwise found in your protein.