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Digestion with MBO1

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#1 epigenetics



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Posted 26 May 2009 - 11:18 AM

I am trying to do digestion with MBO1 on bisulfite modified sample. So did PCR, got single bright band at 150 bp. Then 1 -2 microgm of PCR product was kept with MBO1 at 37 degree for 3/ 4 hrs. It should give me digested bands 110 and 40 bp.
But i am getting same band only at 150 bp after digestion also, no other product.
Infact in gel when i ran digested samples next to undigested one, the digested band appears to be a little bit higher than 150 bp.
Where s the problem. I dont have any idea why digestion is not working. Should i go overnight? Any other suggestions?
Any help will be appreciated.

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