I have undiluted DNA samples and I know the concentration (ng/ul) of each of the samples but I need to dilute them for analysis to between 5-15 ng/ul. Cant remember what solution they mentioned that I will be using for dilution though the question I am asking doesnt really depend on that.
What I need clarified is how I work out how much of the original undiluted DNA sample I need to add to the soln to make it up to the right concentration range. For example, if I have a DNA sample of 1000 ng/ul, how do I work out what volume of this to add to the soln to make its concentration 5-15 ng/ul?
Hopefully I havent confused you all with my wording, thanks in advnace for any help!
Edited by dorts, 26 May 2009 - 07:13 AM.