i´m currently doing some transfection stuff and have to quantify vector as well as mRNA afterwards!
when i run my PCR, signal comes at around Ct 23-26 (means that transfection was OK)!
when i perform DNase I pre-treatment before cDNA-Syntehsis and run real time PCR with my RNA as template, product signals come at 33-35Ct.
Can i be sure that my DNase treatment was efficient enough? is there still DNA contaminating my RNA???
Edited by moljul, 26 May 2009 - 02:38 AM.