Somewhat of a basic question about semantics, but could anyone tell me how a doubling time experiment (to examine the effect of a compound) would differ from a cytotoxicity assay (also examining the effect of a compound)? Are they not the same? Also are doubling time experiment and proliferation assay used interchangably?
Lastly, is it possible to calculate doubling time directly from the absorbances given in MTT/Alamar blue assays? It would seem that the absorbances should directly relate to cell concentrations, so could you just sub in the absorbances for cell concentration in a doubling time equation? Doubling time (hours) = ln2 / ((ln (A/A0)) / t)
Edited by cwong1215, 25 May 2009 - 05:24 PM.