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purifying MMP9


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#1 smilealie

smilealie

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Posted 24 May 2009 - 11:55 PM

hello every one,

i need to purify MMP9 from conditioned media so i need gelatin tagged to sepharose beads. So any one have protocol how gelatin is tagged to sepharose beads.

thanks in advance,
alie.

#2 Telomerase

Telomerase

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Posted 25 May 2009 - 02:06 AM

hello every one,

i need to purify MMP9 from conditioned media so i need gelatin tagged to sepharose beads. So any one have protocol how gelatin is tagged to sepharose beads.

thanks in advance,
alie.


You need to buy pre-made gelatin sepharose 4B amersham beads.
What lab are you from? PM me?
"Beware the power of a PhD student" - scolix




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