Jump to content


- - - - -

MMP 9 isolation using sepharose beads


1 reply to this topic

#1 smilealie

    member

  • Active Members
  • Pip
  • 18 posts

Posted 24 May 2009 - 11:54 PM

hello every one,

i need to purify MMP9 from conditioned media so i need gelatin tagged to sepharose beads. So any one have protocol how gelatin is tagged to sepharose beads.

thanks in advance,
alie.

#2 bob1

    Hmmm, I think it's working

  • Active Members
  • PipPipPipPipPip
  • 3,049 posts

Posted 25 May 2009 - 04:45 PM

Wouldn't it be better to immunoprecipitate with an antibody and protein A or G sepharose beads?





Home - About - Terms of Service - Privacy - Contact Us

©1999-2011 Protocol Online, All rights reserved.