Posted 24 May 2009 - 05:08 AM
I'm working on Lisianthus (plant from the family Gentianaceae).
recently I have a problem of a genomic dna degradation, the DNA was produced using chloroform-octanol it looked O.K at the begging but after a few weeks in -4 it was a complete mess.
any suggestions why it could happen?
what can I do to avoid it?
Posted 24 May 2009 - 04:17 PM
Posted 24 May 2009 - 11:45 PM
the DNA was resuspended in TE buffer and the tubes were autoclaved...
Did you resuspend your DNA in 10 mM tris pH 7.0-7.5 or TE buffer? If you used just water, the DNA will undergo acid hydrolysis, degrading it. Otherwise it could be that you have DNase contamination of your samples (maybe in the tubes).
Posted 25 May 2009 - 04:37 PM
Posted 26 May 2009 - 04:36 AM