I have a couple of very basic questions.. I am working with a couple of cell lines and needing to evaluate the effect of my treatments on the modulation of a targeted set of gene expression. using qRT PCR.
So my question then is, how long and at what concentration should I be exposing my treatments? I've seen people do their treatments at a range of incubation time, but is there a gold standard of the time for gene to be induced? or if there is nothing going on after certain hours, then there will just be no effect? And does the concentration matter?
Another thing is, I have a set of real time PCR data from another lab that I have to interpret, and it was categorized as genes upregulated by one, two and three fold and so on (p<0.05). Is there a general cut off line that can be used to filter these data to 'interesting' set of data to be analyzed?
designing mini array experiment
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