I am having trouble getting my RAW 264.7 cells to be confluent uniformly. They seem to be stacking/growing on top of each other instead of growing side-by-side. I have been seeding flasks with 2 x 10^5 cells. I am using DMEM with 10% FBS, 1% Pen/Strep and 1 % HEPES. Can anyone in this forum help me with this issue.
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Stacking of RAW 264.7 cells
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