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Protein in and out of solution

Started by pras45, May 21 2009 01:04 PM

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#1 pras45

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Posted 21 May 2009 - 01:04 PM

Hi,
My protein seems to ppt @ RT and is fine when I keep it at 4 degree or ice. Can anyone tell me what is going on? And I am planning to do functional assay on it and does this in and out of solution make any difference in the protein integrity and activity.

thanks in advance

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#2 swanny

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Posted 21 May 2009 - 07:25 PM

pras45, on May 22 2009, 07:04 AM, said:

Hi,
My protein seems to ppt @ RT and is fine when I keep it at 4 degree or ice. Can anyone tell me what is going on? And I am planning to do functional assay on it and does this in and out of solution make any difference in the protein integrity and activity.

thanks in advance

What format is your protein in? Buffer? Concentration? Detail, please.

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