Quantifying filamentous bacteria growth
Posted 20 May 2009 - 09:03 PM
I need to look at the growth curve, but normal OD600 analysis won't work due to the filamentous nature of the cells. I've been looking into packed cell density, protein production, DNA quantification and other spectro using the coloured pigments produced by the bacteria. Has anyone used any of these methods or have any other suggestions?
Posted 21 May 2009 - 04:19 PM
Posted 21 May 2009 - 05:53 PM
I have a couple of question: What brand/type of DNA binding dye do you use? Do you quantify your cells using spectro or microscopy?
Posted 03 December 2009 - 11:20 AM
Posted 07 December 2009 - 08:53 AM
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