Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!

Lentivirus transduction of spleen cells

  • Please log in to reply
2 replies to this topic

#1 medo



  • Members
  • Pip
  • 5 posts

Posted 20 May 2009 - 07:16 PM

Hello all,

I am transfecting 293T cells with packaging lentiviral plasmids to generate a lentiviral particles carrying GFP and another gene. When I infect 293T cells with the viral particles to calculate the viral titer I get a very high efficient GFP signal on FACS. But the problem is, when I infect mice spleen cells nothing really happens !. Anybody ever used lentivirus for transduction of cells ? Do you have any suggestions to imprvoe the infection of the cells?

Many thanks.

#2 madrius1



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 114 posts

Posted 21 May 2009 - 06:24 AM


Lentivirus are a pretty strong way to infect cells. That said, I don't think you can compare 293T cells (whitch you could infect by simply looking at them since they are so easy to infect/transfect) and live spleen cells for virus efficiency. You may try to add more virus and check afterwards. It's also important to add 8g/ml of polybrene to your virus : it'll greatly enhance the virus's capacity to bind and infected cells.

Best wishes,

#3 bob1


    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,504 posts

Posted 21 May 2009 - 04:49 PM

You are also crossing species -293 are human, mouse spleen is, well..., mouse. This may have some affect on how well the lentivirus works (I've never used them so I don't know, but typically viruses are reasonably species specific).

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.