Does anyone know the technique to take protein out of the nitrocellose membrane? Would boiling in SDS help? after extraction will the protein be suitable for analysis?
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Posted 20 May 2009 - 09:00 PM
Hey,
Never done it with a Nitrocellulose membrane but you can think on these lines: Cut the band out and put it in a dialysis tubing with some buffer, tie on both ends and subject it to electrophoresis. The protein should come out in the buffer. This surely works for an acrylamide gel.
I doubt if the protein would be good for biochemical analysis but you can try.
Best,
TC
Never done it with a Nitrocellulose membrane but you can think on these lines: Cut the band out and put it in a dialysis tubing with some buffer, tie on both ends and subject it to electrophoresis. The protein should come out in the buffer. This surely works for an acrylamide gel.
I doubt if the protein would be good for biochemical analysis but you can try.
Best,
TC
pras45, on May 21 2009, 02:07 AM, said:
Does anyone know the technique to take protein out of the nitrocellose membrane? Would boiling in SDS help? after extraction will the protein be suitable for analysis?
