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taking protein out of membrane


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#1 pras45

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Posted 20 May 2009 - 12:37 PM

Does anyone know the technique to take protein out of the nitrocellose membrane? Would boiling in SDS help? after extraction will the protein be suitable for analysis?

#2 T C

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Posted 20 May 2009 - 09:00 PM

Hey,

Never done it with a Nitrocellulose membrane but you can think on these lines: Cut the band out and put it in a dialysis tubing with some buffer, tie on both ends and subject it to electrophoresis. The protein should come out in the buffer. This surely works for an acrylamide gel.

I doubt if the protein would be good for biochemical analysis but you can try.

Best,
TC

Does anyone know the technique to take protein out of the nitrocellose membrane? Would boiling in SDS help? after extraction will the protein be suitable for analysis?






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