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Genomic Contig


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#1 Sam80

Sam80

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Posted 20 May 2009 - 02:51 AM

I am new to the world of PCR so please excuse my ignorance!

I recently ran a RT-PCR and sent the PCR product off for sequencing, to find out if I had indeed amplified my intended target! On receipt of the sequence I did a blast search and it came back that my sequence matched 96% with homo sapiens chromosome 15 genomic contig featuring chondroitin sulphate proteoglycan 4 (NG2). Could someone please explain what this means? I was looking to amplify NG2 - so does this mean the RT-PCR has worked?

Many thanks,
Sam




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