hey all,
i am doing western blots and for my loading control (actin), i am consistently getting a specific band at 55-60 kd (the expected size is 42 kd). these are 293 cells that have been infected with adenovirus containing a gene of interest. im having trouble explaining why my size is off. any thoughts?
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#2
Curtis
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Posted 19 May 2009 - 02:20 AM
my actin also comes down to 50-60KDa...I bought from Sigma.
#3
medstudent1
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Posted 19 May 2009 - 10:21 AM
Curtis, on May 19 2009, 02:20 AM, said:
my actin also comes down to 50-60KDa...I bought from Sigma.
interesting. mine is from sigma as well (monoclonal actin). any explanation as to what it is running higher? bound to another protein? post-translational modifications? what cell line are you using.
#4
bob1
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Posted 20 May 2009 - 05:28 PM
We use Abcam A16 actin, it runs at about 46 kDa and is very strong. The ab we had from before (either sigma or santa cruz?) was a goat and very dirty with quite a few non-specific bands.
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medchemgirl
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Posted 15 June 2009 - 12:22 PM
This is good to know!
