actin MW problem in western blot
Posted 18 May 2009 - 10:19 AM
i am doing western blots and for my loading control (actin), i am consistently getting a specific band at 55-60 kd (the expected size is 42 kd). these are 293 cells that have been infected with adenovirus containing a gene of interest. im having trouble explaining why my size is off. any thoughts?
Posted 19 May 2009 - 02:20 AM
Posted 19 May 2009 - 10:21 AM
my actin also comes down to 50-60KDa...I bought from Sigma.
interesting. mine is from sigma as well (monoclonal actin). any explanation as to what it is running higher? bound to another protein? post-translational modifications? what cell line are you using.
Posted 20 May 2009 - 05:28 PM