3 replies to this topic

[dvddecarvalho]

Dear all,
I am having problems to produce a good titer of viral particules by using Phoenix amphopac cells.
A friend of mine told to select the cells with hygromycin and diphtheria toxin as the viral proteins and envelop are cloned in this cells in the same cluster of this resistance genes.
What do you guys thing abou it?
Is there another drug to substitute the diphtheria toxin as long as is a very difficult to find it?

Thanks,
Deivid

[Clare]

Hello

Yes, we made sure we selected our cells. Use 1ug/ml diphtheria toxin (Sigma) and 500ug/ml hygromycin (Sigma) for 2 weeks.
Hope this helps
Clare

dvddecarvalho, on May 18 2009, 06:20 PM, said:

Dear all,
I am having problems to produce a good titer of viral particules by using Phoenix amphopac cells.
A friend of mine told to select the cells with hygromycin and diphtheria toxin as the viral proteins and envelop are cloned in this cells in the same cluster of this resistance genes.
What do you guys thing abou it?
Is there another drug to substitute the diphtheria toxin as long as is a very difficult to find it?

Thanks,
Deivid

[dvddecarvalho]

Ok,
Thank you Clare.
Is necessary special care in the manipulation of the diphtheria toxin?
I mean special handling, wasting, etc.

Thanks again

[Clare]

Just check the MSDS and product info

Clare

dvddecarvalho, on May 19 2009, 03:37 PM, said:

Ok,
Thank you Clare.
Is necessary special care in the manipulation of the diphtheria toxin?
I mean special handling, wasting, etc.

Thanks again