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Phoenix packaging cell and diphtheria toxin


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3 replies to this topic

#1 dvddecarvalho

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Posted 18 May 2009 - 09:20 AM

Dear all,
I am having problems to produce a good titer of viral particules by using Phoenix amphopac cells.
A friend of mine told to select the cells with hygromycin and diphtheria toxin as the viral proteins and envelop are cloned in this cells in the same cluster of this resistance genes.
What do you guys thing abou it?
Is there another drug to substitute the diphtheria toxin as long as is a very difficult to find it?

Thanks,
Deivid

#2 Clare

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Posted 19 May 2009 - 06:12 AM

Hello :)

Yes, we made sure we selected our cells. Use 1ug/ml diphtheria toxin (Sigma) and 500ug/ml hygromycin (Sigma) for 2 weeks.
Hope this helps B)
Clare



Dear all,
I am having problems to produce a good titer of viral particules by using Phoenix amphopac cells.
A friend of mine told to select the cells with hygromycin and diphtheria toxin as the viral proteins and envelop are cloned in this cells in the same cluster of this resistance genes.
What do you guys thing abou it?
Is there another drug to substitute the diphtheria toxin as long as is a very difficult to find it?

Thanks,
Deivid



#3 dvddecarvalho

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Posted 19 May 2009 - 06:37 AM

Ok,
Thank you Clare.
Is necessary special care in the manipulation of the diphtheria toxin?
I mean special handling, wasting, etc.

Thanks again

#4 Clare

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Posted 20 May 2009 - 06:59 AM

Just check the MSDS and product info :D

Clare

Ok,
Thank you Clare.
Is necessary special care in the manipulation of the diphtheria toxin?
I mean special handling, wasting, etc.

Thanks again






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