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His Tag protein


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4 replies to this topic

#1 FRAROAR

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Posted 18 May 2009 - 03:54 AM

Good morning dear all, I want to espress a big protein..about 1070 aa.
I want to insert six His to this protein to improve the purification. are suffient 6 His or the protein is to big to purify with a Ni column?

Thanks,

Fra

#2 Vini

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Posted 18 May 2009 - 04:33 AM

Good morning dear all, I want to espress a big protein..about 1070 aa.
I want to insert six His to this protein to improve the purification. are suffient 6 His or the protein is to big to purify with a Ni column?

Thanks,

Fra



that should not be a problem. 6 His residues are sufficient...

#3 Curtis

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Posted 18 May 2009 - 06:01 AM

how can you add 6 His?

why not use a commercially available his-tag vector. what would be the difference?

#4 Vini

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Posted 18 May 2009 - 06:13 AM

how can you add 6 His?

why not use a commercially available his-tag vector. what would be the difference?



i guess FRA meant using a his-tag vector only, just simplified the sentence! B)

#5 T C

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Posted 18 May 2009 - 03:05 PM

Hey,

Prefer a Cter His tag as that will ensure that no truncated forms of the proein purify.

Best,
TC




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