Hi all,
is it possible to prepare somehow even volumes of different samples with different concentrations each in order to avoid adjusting the pipet each time?
I have more tha 10 samples and the adjusting thing each time kills me and besides I dont like leaving the protein in the WB gel for long cause it starts migrating in the gel and that gives a dirty appearance of my gel after the run
thx a lot
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3 replies to this topic
#2
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Missele, the little mouse
Posted 18 May 2009 - 12:37 AM
calculate the concentration of your samples, and dilute them so they have the same final concentrations. V2 = C1*V1/C2
#3
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Enthusiast
Posted 18 May 2009 - 09:34 AM
little mouse, on May 18 2009, 01:37 AM, said:
calculate the concentration of your samples, and dilute them so they have the same final concentrations. V2 = C1*V1/C2
Hey little mouse, thx for the tip. Dilute them with what? dH20, Lysis buffer? what exactly?
#4
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Hmmm, I think it's working
Posted 18 May 2009 - 05:22 PM
lysis buffer or other neutral solution
