Even volumes of different samples
Posted 17 May 2009 - 04:57 PM
is it possible to prepare somehow even volumes of different samples with different concentrations each in order to avoid adjusting the pipet each time?
I have more tha 10 samples and the adjusting thing each time kills me and besides I dont like leaving the protein in the WB gel for long cause it starts migrating in the gel and that gives a dirty appearance of my gel after the run
thx a lot
Posted 18 May 2009 - 12:37 AM
Posted 18 May 2009 - 09:34 AM
calculate the concentration of your samples, and dilute them so they have the same final concentrations. V2 = C1*V1/C2
Hey little mouse, thx for the tip. Dilute them with what? dH20, Lysis buffer? what exactly?