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Even volumes of different samples


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3 replies to this topic

#1 Aris

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Posted 17 May 2009 - 04:57 PM

Hi all,

is it possible to prepare somehow even volumes of different samples with different concentrations each in order to avoid adjusting the pipet each time?
I have more tha 10 samples and the adjusting thing each time kills me and besides I dont like leaving the protein in the WB gel for long cause it starts migrating in the gel and that gives a dirty appearance of my gel after the run

thx a lot

#2 little mouse

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Posted 18 May 2009 - 12:37 AM

calculate the concentration of your samples, and dilute them so they have the same final concentrations. V2 = C1*V1/C2

#3 Aris

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Posted 18 May 2009 - 09:34 AM

calculate the concentration of your samples, and dilute them so they have the same final concentrations. V2 = C1*V1/C2


Hey little mouse, thx for the tip. Dilute them with what? dH20, Lysis buffer? what exactly?

#4 bob1

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Posted 18 May 2009 - 05:22 PM

lysis buffer or other neutral solution




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