G1= chromosomal DNA from Vibrio fischeri
G2= chromosomal DNA from Vibrio fischeri cut with Sal I restriction enzyme to obtain the lux operon
P1= plasmid DNA from Escherichia coli pUC18
P2= plasmid DNA from Escherichia coli pUC18 cut with Sal I restriction enzyme
why some is lower some is higher?
i know smaller size of dna can travel further and uncut DNA is travel further than the DNA cut with Sal I, but the results doesnt match
G1 suppose to be lower then G2 as it is un cut..... any body can help me?
i still got 2 more questions... What is the range of restriction enzyme fragment sizes gernerated from Sal I digestion of Vibrio fischeri DNA.. is the question asking that they want to know the Sal I enzyme cut which part of the DNA? or the size of the DNA after cutting with Sal I.
2nd) As Sal I recognize a 6 base sequence, what is the theireutcak size distribution you would expect.? is the question asking how big ish the size of the enzyme?
lastly thanks in advance (sorry for the bad photo)
Thanks in advance to those who can help
Edited by silentears, 16 May 2009 - 05:57 PM.