293s, transfection and MTT assay
Posted 15 May 2009 - 11:11 PM
My problem is that whenever i do the transfection, I have to change the media in the wells before I add the transfection mixture (I use Fugene), I noticed that after the media change I lost many cells in the wells. I understand that 293s are loosely adherent. I believe the lost of the cells have ultimately caused the inconsistent readings that I get from the MTT assay.
Should I coat the well with poly-D-lysine before seeding the cells? I've been just removing the media by pipetting, which may have dislodge the cells? Can anyone give me some advice on how to go about with the experiment?
Posted 17 May 2009 - 04:26 PM
Posted 18 May 2009 - 06:20 PM
Posted 19 May 2009 - 04:34 PM