Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

protein extraction after cell lysing


  • Please log in to reply
2 replies to this topic

#1 abohollolo

abohollolo

    member

  • Active Members
  • Pip
  • 20 posts
0
Neutral

Posted 15 May 2009 - 12:16 AM

i want to extract collagen from tissue implants. to measure its concentration later.

first i need to homogenize the tissue
then i need to lyse the cell and measure total protein content for nromalization of the collagen content i will measure later.

the questions are : 1- where is my protein (collagen) located after lysing ( in the pellet or in the supernatant ) ?
2- if collagen is in the supernatant how do i switch from the homogenzation solution (cell lysing buffer + protease inhibator ) to the exctraction solution (acetic acid + pepsin) . i was thinking by lyophelize ?!

thanks for help

#2 maha

maha

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 28 May 2009 - 12:17 AM

Proteins (also known as polypeptides) are organic compounds made of amino acids arranged in a linear chain polymer and joined together by peptide bonds between the carboxyl and amino groups of adjacent amino acid residues.
_______________________________________________________________________________
Børnetøj
sexy lingerie

#3 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,728 posts
399
Excellent

Posted 28 May 2009 - 04:43 PM

Step one...go Here.
Step two...read.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.