Do you lose RNA by quick freezing?
Posted 14 May 2009 - 01:03 PM
Posted 14 May 2009 - 04:17 PM
Posted 14 May 2009 - 09:04 PM
Posted 15 May 2009 - 12:40 AM
many people store RNA after snap freezing. I cant imagine what could be the problem with that!!!
Posted 15 May 2009 - 03:40 AM
1. RNAlater and processed sstraight away (best RNA quality)
2. RNAlater and frozen/snap frozen
3. Snap frozen in nitrogen
4. Frozen tissue, no nitrogen.
REally it depends what you want to use the RNA from the tissue for, but if you wanted it for something as accurate as microarray analysis, i would reccomend some kind of RNA preservative like RNAlater (qiagen) though lots of companies have their own versions.
Posted 18 May 2009 - 03:47 AM