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Can I perform Dig-labelled ISH on paraffine sections?


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#1 Juan Martinez

Juan Martinez

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Posted 27 January 2002 - 06:51 AM

I would like to obtain information about your experience performing in situ hybridisation in paraffine sections: not with radioactive labelled probes, but with digoxigenin or biotinilated probes. The point is that the protocols for frozen secctions does not semms to work with the paraffine ones. Thank you for the help.




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