Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Secondary Antibody: adsorbed?


  • Please log in to reply
1 reply to this topic

#1 culturelady

culturelady

    member

  • Active Members
  • Pip
  • 11 posts
0
Neutral

Posted 14 May 2009 - 05:57 AM

Hi everyone,

I'm quite new to the staining area and need to buy a secondary antibody, but if the product description says it has been "adsorbed" against rat IgG, does that mean I cant use it against cells derived from rat?

So confused!!

Thanks guys :D

#2 sgt4boston

sgt4boston

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 276 posts
1
Neutral

Posted 14 May 2009 - 07:40 AM

Usually product description will indicate specificity and adsorption against other animal products to remove any cross reactive abs that may exist.

For example adsorbed against rat IgG means product should not react with rat IgG.

I prefer to use products that state specificity AND "no reaction" with.... <animal, antibody class, light chain...>




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.