Sterilize organic solvents
Posted 14 May 2009 - 05:42 AM
Posted 14 May 2009 - 06:46 AM
Posted 14 May 2009 - 07:02 AM
Posted 14 May 2009 - 07:39 AM
don't try to filter sterilize DMSO, it will dissolve the glue that sticks the different parts of the filter.
But you can filter your medium or buffer after addition of DMSO.
Posted 14 May 2009 - 09:26 AM
You should definitely filter sterilize, and as LM suggests organics can cause problems with certain filters. You just have to be careful to use the filters compatible with your solvent of choice. We routinely use [url="http://labfilters.pall.com/catalog/laboratory_20018.asp"]DMSO safe filters [/url]available from Pall to sterilize DMSO and MeH/EtOH solutions.
Posted 14 May 2009 - 09:44 AM
For EtOH and MeOH, no need either. Nothing will grow in these solvants.
Posted 14 May 2009 - 10:19 AM
I would just dissolve your compounds, store them for at least 30 minutes and then use.
If you feel that you must filter-sterilize remember to use a solvent-resistant filter!
Hope this helps
Posted 14 May 2009 - 04:47 PM
Posted 14 May 2009 - 09:26 PM
Just freshly distill the solvents and use. And avoid plastic when using solvents, solvents will leach out plastizers.