I am checking the Mdr expression level in two cancer cell lines by WB using a monoclonal antibody from SCBT. There is a very beautiful band, very specific(?), but the size is about 60KDa, not the predicted 190KDa. If degraded during preparation of cell lysates, it is unlikely that there is only one sharp band on all of the samples (total 8 with different treatments). I called the tech support of SCBT. They gave me a polyclonal antibody to test. There is a prominent band of 60KDa, with many weak (unspecific?) bands. Does anybody have an idea what wrong with my WB?
Any suggestions are appreciated.
Edited by hepliu, 13 May 2009 - 12:51 PM.