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TOPO cloning problem

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15 replies to this topic

#16 hanming86



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Posted 28 May 2009 - 08:16 PM

I suspect there's somehting wrong with he TOPO reaction or the lack of A-tail. TOP10 cell is pretty robust. and u can easily make them chemically or electrocompetent. since the control is working, that means the cells are ok. they can take abit of "less-gentle" treatment. either that or ur clone is toxic to the cell.
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