Method for lysing cells for fractionation
Posted 13 May 2009 - 06:55 AM
Posted 13 May 2009 - 07:00 AM
then heaven will be yours, before you meet your end
Posted 13 May 2009 - 12:14 PM
Posted 13 May 2009 - 07:10 PM
If you have access to liquid nitrogen, a few freeze-thaw cycles (preferably in lysis buffer) should work fine. Dry ice may work too.
Thanks for the suggestion. We have liquid nitrogen in the lab, but won't freeze-thaw distrupt also the membranes of the organelles, eg. mitochondria?
Posted 13 May 2009 - 07:13 PM
Most effective would be using a french press if you have access to one...
Thanks for the suggestion but we don't have a french press in the lab. Is it OK to pass my cells in a syringe instead? I just want to distrupt the plasma membrane and not organellar membranes. Thanks again.