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sensitive protein quantification assay


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#1 cupidstunt

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Posted 13 May 2009 - 06:47 AM

Hi!

I am extracting membrane protein (tagging and extracting) from cells and I am facing problem quantifying them. I use BCA and my standard curve is ok. I use 1mg/ml BSA in my respective buffer as my standard. and use diff concs. I also tried eluting my protein using less eluting buffer, but didnt help. I think BCA is not very sensitive in my case. I also made sure all components being used are compatible with the assay.

Please suggest help!!

THanks rolleyes.gif

#2 bob1

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Posted 13 May 2009 - 04:19 PM

You can't detect the protein by BCA or is it giving you variable readings?

#3 cupidstunt

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Posted 14 May 2009 - 01:02 AM

You can't detect the protein by BCA or is it giving you variable readings?

cant detect!

#4 sgt4boston

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Posted 14 May 2009 - 07:47 AM

Can you take a small aliquot and concentrate?

Do you have a "membrane" protein available to use as a control in the BCA?




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