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sensitive protein quantification assay


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2 replies to this topic

#1 cupidstunt

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Posted 13 May 2009 - 06:45 AM

Hi!

I am extracting membrane protein (tagging and extracting) from cells and I am facing problem quantifying them. I use BCA and my standard curve is ok. I use 1mg/ml BSA in my respective buffer as my standard. and use diff concs. I also tried eluting my protein using less eluting buffer, but didnt help. I think BCA is not very sensitive in my case. I also made sure all components being used are compatible with the assay.

Please suggest help!!

THanks <_<

#2 sgt4boston

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Posted 15 May 2009 - 06:33 AM

Concentrate sample? Verify performance/sensitivity of the assay with control material.

#3 mdfenko

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Posted 15 May 2009 - 10:52 AM

have you tried the bradford protein assay?
talent does what it can
genius does what it must
i do what i get paid to do




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