huyentran, on 12 February 2010 - 04:27 AM, said:
huni, on Jan 5 2010, 05:53 AM, said:
Do you guys know this paper published in 2009 titled as 'A simple and loss-free method to remove TRIzol contaminations from minute RNA samples'?
At least I observed that the RNA purity increased a lot after purification following this simple and cheap protocol.
Link is below;
I did follow the protocol in this paper, however I still have low 260/230 ratio (below 1) and that is not good for labelling (Affymetrix array). Can anyone please give me some advice to improve 260/230. My samples are monocytes from clinical samples. They are very "tiny" samples and precious. Please help. Thank you.
Hi, I am doing a lot of microarrays with affymetrix array, and I always purify my trizole purified RNAs with the above mentioned method.
Even though sometimes samples have a low 260/230 ratio, my microarry amplication were always successful with this purification method-Until now, 100%.
I am replying to you to let you know my experience with this method for consideration.