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Nuclease acivity of metagenomic DNA

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#1 meshohel



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Posted 12 May 2009 - 08:26 PM

I tried to construct fosmid library from the metagenomic DNA. But whenever I try to isolate DNA the size of the DNA is close to 20-25 kb.
But I need 40kb. Can it be because of nuclease activity?? If so, how could I solve this problem.

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