Can DNA be solubilized in hydrochloric acid?
Posted 12 May 2009 - 08:15 PM
What I would like to do is to normalize the amount of calcium to the amount of DNA present. Would the HCl be able to solubilize the DNA from the cells, and hence I can measure the DNA in the supernatant using some sort of fluorescent nucleic acid dye (e.g. DAPI)?
Or if someone has any other protocol to measure matrix calcium and DNA in the same sample it'd be greatly appreciated!
Posted 13 May 2009 - 04:26 AM
Posted 13 May 2009 - 05:43 PM