convex and concave 24-well plate
Posted 12 May 2009 - 05:51 AM
Comparing to the one we used in the past, the new 24-well is a little convex in the middle of the wells and the used 24-well is concave!
Dose anyone know the reason for the convex or concave design?
Use the convex 24-well, it would disperse the cell around the circle of wells.
Use the concave one, it would focus the cell on the center of wells.
I even don't know how much cell amount I should add for optimal 50~60% conflux of transfection (in our lab, we don't count cell, the adding amount according to our own sense), because the cell would not attach to wells averagely.
Why can't it be designed as flat as T-flask is?
Because after EGFP-plasmid transfection into cell, I detect flurorescent of the plate directly by 488nm excitation and 507nm emission.
Dose the convex or concave design affect the result of detection of excitation and emssion?
Posted 12 May 2009 - 05:48 PM
Understanding Life One Protein At a Time...
A peptide synthesis company.
Posted 12 May 2009 - 05:49 PM
The only way to accurately and reproducibly do experiments is to count your cells for confluency estimation at seeding. If you are not doing this then you are failing one very important consideration in the scientific method - reproducibility. It is OK to have different cell numbers for different size cells (e.g neuron vs colon epithelial), so long as within one cell line you are consistent.