Posted 31 May 2002 - 10:00 AM
Posted 01 June 2002 - 03:28 AM
I don't really understand the bands you are talking about...after digestion of the genomic dna usually you obtain something like a smear. Is your digestion really efficient?? (ie: 2h @ 37°C, add again enz and then ON @ 37°C for a complete digest).
Posted 01 June 2002 - 04:24 AM
In my digestion (with XmaI) appears some bands and a smear, but this bands appear ever (I did it 2 times). And are those band I saw in the film. Why?
Posted 01 June 2002 - 09:59 AM