Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

is hot weather a important factor in RNA extraction?


  • Please log in to reply
1 reply to this topic

#1 salmon667

salmon667

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 05 June 2002 - 05:23 AM

Does the degrade of Exracted RNA have a close relationship with this hot weather?

#2 milanoj

milanoj

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 72 posts
0
Neutral

Posted 05 June 2002 - 08:02 AM

I've found that it's not so much the hot weather but the phases of the moon. In my experience when the moon is waxing my RNA preps are intact. Unfortunately, when the moon is waning my RNA is usually degraded except in the fall when results may vary.
Seriously, your RNA is probably the victim of RNAse. The warmer the ambient temperature the more active the RNAse is degrading your RNA. If you can resuspend the final pellet in a stabilized formamide product called Formazol made by the same people that make RNAzol.
Good Luck and remember only prepare RNA when the moon is waxing.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.