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Met trouble in ligation!

Started by Hans Lee, Mar 30 2002 07:56 PM

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#1 Hans Lee

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Posted 30 March 2002 - 07:56 PM

insert:sacII,XhoI;vector:sacII,SalI, is there any good method to improve this ligation ? XhoI and SalI have the same 5' terminal after digestion.
medbee@chinaren.com

#2 Yifan Lu

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Posted 15 June 2002 - 06:47 AM

XhoI and SalI are blunted with T4 DNA polymerase
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