4 replies to this topic

[waichun]

I have to miniprep some sample from which the bacterial pellets have been stored for more than 6 months at -20. I've found some of them hard to resuspend even after vortexing and heating up to 37.
do you have any suggestion?

[perneseblue]

waichun, on May 7 2009, 08:00 AM, said:

I have to miniprep some sample from which the bacterial pellets have been stored for more than 6 months at -20. I've found some of them hard to resuspend even after vortexing and heating up to 37.
do you have any suggestion?

vortex the pellet before adding any solutions. It is easier to break up the pellet before adding the cell lysis solution.

[LifeTein Peptide]

You can freeze and thaw several times before you do the miniprep directly.


James
Understanding Life One protein at a time...
www.lifetein.com

[Lazinase]

Tell you one trick that I use...use the pipette to resuspend the pellet as vortexing takes forever...

[waichun]

pipette up and down works. thank you