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Resuspend bacterial pellet


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4 replies to this topic

#1 waichun

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Posted 07 May 2009 - 08:00 AM

I have to miniprep some sample from which the bacterial pellets have been stored for more than 6 months at -20. I've found some of them hard to resuspend even after vortexing and heating up to 37.
do you have any suggestion?

#2 perneseblue

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Posted 07 May 2009 - 08:27 AM

I have to miniprep some sample from which the bacterial pellets have been stored for more than 6 months at -20. I've found some of them hard to resuspend even after vortexing and heating up to 37.
do you have any suggestion?


vortex the pellet before adding any solutions. It is easier to break up the pellet before adding the cell lysis solution.
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#3 LifeTein Peptide

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Posted 07 May 2009 - 08:50 AM

You can freeze and thaw several times before you do the miniprep directly.


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#4 Lazinase

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Posted 07 May 2009 - 10:12 PM

Tell you one trick that I use...use the pipette to resuspend the pellet as vortexing takes forever...

#5 waichun

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Posted 12 May 2009 - 07:48 AM

pipette up and down works. thank you




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