I'm trying to amplify some patient sample for a specific location. Before doing that, I tested the condition on the lab strain, NL4-3, which gave me a sharp band on the gel. However, when I used the same setting on patient sample, despite of the desired band, there were also some non-specific binding bands...so except increase Tm, decrease primer conc., what else I should do?
Any suggestions is welcome and appreciated.
Edited by Lazinase, 06 May 2009 - 07:33 PM.