11 replies to this topic

[Neurite]

Hello all...

Could somebody tell me, the differences between DMEM medium and DMEM F12 !? As silly as it will sound, I cant find it anywhere....    

Thank you in advance !!!

[bob1]

DMEM:F12 is DMEM mixed 1:1 with F12 medium.

[Neurite]

And the F12 medium is....... ?

[cotchy]

Neurite, on May 7 2009, 03:45 AM, said:

And the F12 medium is....... ?

F 12 is also called Hams F12, any media supplier will stock this and also have the exact recipe for it

[Neurite]

My friend gave me some cells that were in DMEM/F12, yesterday I just put them in DMEM.... they are fine today  

[hidayahh]

i'm now grow the osteoblast hfob 1.19 and osteosarcoma  U2-OS. before this i gave them DMEM/F12 (together with FBS and penstrep). DMEM/F12 already finished but i m still need to grow them. Can i use DMEM without F12 to grow them? I have DMEM only for this time. Can anyone response me?

[bob1]

hidayahh, on 26 November 2012 - 08:08 PM, said:

i'm now grow the osteoblast hfob 1.19 and osteosarcoma  U2-OS. before this i gave them DMEM/F12 (together with FBS and penstrep). DMEM/F12 already finished but i m still need to grow them. Can i use DMEM without F12 to grow them? I have DMEM only for this time. Can anyone response me?

This is a tricky one - the cells will probably grow fine in DMEM, but (and it is a big BUT) how will this affect your experiments and how will these results compare with results you already have?  There are likely to be quite a number of gene expression changes with the medium change, so you would need to check very carefully on the growth rates and any genes that you are interested in looking at.

[hidayahh]

oh yes! thank you for the reminder. very useful information. i love my cells, and its gene content.  so i need to stop for a while and cryopreserve them first in order to wait for the new media dmem/f12. is that ok?

[rhombus]

Neurite, on 06 May 2009 - 02:14 PM, said:

Hello all...

Could somebody tell me, the differences between DMEM medium and DMEM F12 !? As silly as it will sound, I cant find it anywhere....

Thank you in advance !!!

Dear Neurite,

If you are in the UK then follow:

Invitrogen website  -  Products and Services - Cell Culture - mammalian cell culture - DMEM  DMEM/F12

The full ingredients are listed as above but if you are outside the UK then these are the relevant links:-


http://www.invitroge...lation.170.html



http://www.invitroge...ulation.55.html


Hope you find this useful

Kindest regards

Uncle Rhombus

[bob1]

hidayahh, on 27 November 2012 - 03:04 AM, said:

oh yes! thank you for the reminder. very useful information. i love my cells, and its gene content.  so i need to stop for a while and cryopreserve them first in order to wait for the new media dmem/f12. is that ok?

Yes it is fine to freeze them down.

[hidayahh]

thank you for your confirmation about the freezing ;

what if i used dmem/f12 with phenol red then due to some problem, i used the same media but without phenol red. is that any risk ?

[bob1]

Potentially - phenol red is a weak estrogen analogue...