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DMEM vs. DMEM F12


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#1 Neurite

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Posted 06 May 2009 - 02:14 PM

Hello all...

Could somebody tell me, the differences between DMEM medium and DMEM F12 !? As silly as it will sound, I cant find it anywhere.... :rolleyes:

Thank you in advance !!!

#2 bob1

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Posted 06 May 2009 - 04:29 PM

DMEM:F12 is DMEM mixed 1:1 with F12 medium.

#3 Neurite

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Posted 06 May 2009 - 06:45 PM

And the F12 medium is....... ?

#4 cotchy

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Posted 07 May 2009 - 03:21 AM

And the F12 medium is....... ?


F 12 is also called Hams F12, any media supplier will stock this and also have the exact recipe for it

#5 Neurite

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Posted 07 May 2009 - 07:17 AM

My friend gave me some cells that were in DMEM/F12, yesterday I just put them in DMEM.... they are fine today :D

#6 hidayahh

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Posted 26 November 2012 - 08:08 PM

i'm now grow the osteoblast hfob 1.19 and osteosarcoma U2-OS. before this i gave them DMEM/F12 (together with FBS and penstrep). DMEM/F12 already finished but i m still need to grow them. Can i use DMEM without F12 to grow them? I have DMEM only for this time. Can anyone response me?

#7 bob1

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Posted 26 November 2012 - 11:55 PM

i'm now grow the osteoblast hfob 1.19 and osteosarcoma U2-OS. before this i gave them DMEM/F12 (together with FBS and penstrep). DMEM/F12 already finished but i m still need to grow them. Can i use DMEM without F12 to grow them? I have DMEM only for this time. Can anyone response me?

This is a tricky one - the cells will probably grow fine in DMEM, but (and it is a big BUT) how will this affect your experiments and how will these results compare with results you already have? There are likely to be quite a number of gene expression changes with the medium change, so you would need to check very carefully on the growth rates and any genes that you are interested in looking at.

#8 hidayahh

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Posted 27 November 2012 - 03:04 AM

oh yes! thank you for the reminder. very useful information. i love my cells, and its gene content. so i need to stop for a while and cryopreserve them first in order to wait for the new media dmem/f12. is that ok?

#9 rhombus

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Posted 27 November 2012 - 03:37 AM

Hello all...

Could somebody tell me, the differences between DMEM medium and DMEM F12 !? As silly as it will sound, I cant find it anywhere.... :rolleyes:

Thank you in advance !!!


Dear Neurite,

If you are in the UK then follow:

Invitrogen website - Products and Services - Cell Culture - mammalian cell culture - DMEM DMEM/F12

The full ingredients are listed as above but if you are outside the UK then these are the relevant links:-


http://www.invitroge...lation.170.html



http://www.invitroge...ulation.55.html


Hope you find this useful

Kindest regards

Uncle Rhombus

#10 bob1

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Posted 27 November 2012 - 12:21 PM

oh yes! thank you for the reminder. very useful information. i love my cells, and its gene content. so i need to stop for a while and cryopreserve them first in order to wait for the new media dmem/f12. is that ok?

Yes it is fine to freeze them down.

#11 hidayahh

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Posted 27 November 2012 - 11:02 PM

thank you for your confirmation about the freezing ;

what if i used dmem/f12 with phenol red then due to some problem, i used the same media but without phenol red. is that any risk ?

#12 bob1

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Posted 28 November 2012 - 12:48 AM

Potentially - phenol red is a weak estrogen analogue...

#13 hidayahcellculture

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Posted 17 July 2013 - 09:43 PM

Potentially - phenol red is a weak estrogen analogue...


what is meant by weak estrogen analogue?

#14 bob1

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Posted 18 July 2013 - 01:28 AM

A weak estrogen analogue is a substance that activates the estrogen receptor and will cause internalization etc. THe weakness comes from needing quite a bit of the phenol red there for this to happen. Most phenol-red containing mediums have enough to activate the receptor, so if you are studying the effects of estrogen or something that may be affected by estrogen (breast cells, ovaries etc.), use phenol-red free medium.




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