Protocol for staining live cells
Posted 06 May 2009 - 09:23 AM
Does anyone have a protocol for staining live cells with antibodies against antigens presented on the surface?
Also, on a side note, someone told me that formaldehyde can quenche fluorescence of some fluorophores. Is this true?
And something else, I like to use propidium iodide for staining DNA (mainly for cell cycle analysis). I get weird results if I formaldehyde fix. Does anyone know why?
Posted 06 May 2009 - 12:24 PM
for general protocols, BD Pharmingen's website is very informative and can give you a place to start, based on your cell type and what you are trying to find. they also have great antibodies
Posted 08 June 2009 - 06:54 AM
For which surface antigens to you want to stain?